Learn how to write a SOP for operation of FTIR Spectrometer in pharmaceutical laboratory.
1. PURPOSE
To describes the proper methods for operating the FTIR spectrometer (Fourier Transform Infrared) in laboratory.
2. SCOPE
This SOP is applicable for all personnel trained and authorized to operate the FTIR apparatus.
3. RESPONSIBILITIES
3.1 Executive/Analyst is responsible for following this SOP to ensure correct usage and obtaining valid results and must have training in FTIR operation.
3.2 Manager Quality Control ensures that analysts are properly trained and supervise the operation.
3.3 Maintenance Technician: Responsible for routine servicing, calibration, and troubleshooting of the FTIR spectrometer.
4. ACCOUNTABILITY
Head of the Quality Control Department
5. ASSOCIATED DOCUMENTS / REFERENCES
5.1 Manufacturer’s FTIR user manual.
5.2 Relevant SOPs and protocols of the laboratory.
6. PRECAUTIONS
6.1 Always wear personal protective equipment (PPE), including gloves, lab coat, and safety goggles.
6.2 Ensure that the sample being analyzed does not produce hazardous fumes or vapors.
6.3 If analyzing gases, ensure proper ventilation or perform the analysis in a fume hood.
6.4 Avoid exposure to direct light from the laser in the FTIR.
7. PROCEDURE
7.1 Pre-Operation Setup
7.1.1 Switch on the FTIR spectrometer using the power switch button on the device.
7.1.2 Wait for the system to complete its self-check and initialization sequence.
7.2 Prepare the sample
7.2.1 For solids: Place the sample in the sample holder. If uses ATR then assure direct contact between the sample and the ATR crystal.
7.2.2 For liquids: Prepare the liquid in a thin film or use a liquid cell and conform that the sample is free from bubbles or impurities.
7.2.3 For gases: Ensure the sample is properly introduced into the gas cell, and purge the system with nitrogen if necessary.
7.3 If needed, perform calibration using a standard reference material (e.g., polystyrene or KBr).
7.4 Software Setup
7.4.1 Open the FTIR software on the connected computer.
7.4.2 Verify the system parameters, such as resolution, scanning range (usually 4000–400 cm⁻¹), and the number of scans.
7.4.3 Ensure the baseline is correct by performing a blank scan with no sample in place.
7.5 Sample Analysis
7.5.1 Place the sample into the sample compartment. Ensure it is properly aligned and positioned.
7.5.2 In the FTIR software, click the “Start Scan” button.
7.5.3 The FTIR will collect data by scanning the sample and creating an interferogram.
7.6 Data Collection
7.6.1 Monitor the progress of the scan on the software interface.
7.6.2 The FTIR system will generate a spectrum once the scan is complete. This spectrum represents the absorption of infrared light by the sample at various wavelengths.
7.6.3 Save the spectrum to the appropriate directory on the computer.
7.6.4 Analyze the spectrum using the software. Compare the absorption peaks to known references or databases to identify the chemical components in the sample.
7.6.5 Store all results in the designated folder or database, ensuring proper naming conventions are used for traceability.
7.7 Post-Operation
7.7.1 After the analysis, remove the sample and clean the sample holder or ATR crystal with a lint-free tissue or appropriate cleaning solution. Ensure that no residues remain that could affect future analyses.
7.7.2 Close the FTIR software.
7.7.3 Turn off the FTIR spectrometer following the correct shutdown process.
7.8 Troubleshooting
7.8.1 For low or no signal in the spectrum check the sample placement, ensure the sample is not too thick or too thin, and verify the optical alignment.
7.8.2 For irregular or noisy spectrum increase the number of scans to improve signal-to-noise ratio, clean the ATR crystal or sample holder, and ensure no contamination.
8. DEFINITIONS AND ABBREVIATIONS
8.1 FTIR Spectroscopy: A technique that collects high-resolution spectral data in the infrared region of the electromagnetic spectrum by recording the absorbance of infrared light passing through a sample.
8.2 Sample: The material or substance being analyzed, either solid, liquid, or gas.
8.3 FTIR: Fourier Transform Infrared
8.4 ATR: Attenuated Total Reflectance
8.5 QC: Quality Control
8.6 PPE: Personal Protective Equipment
8.7 SOP: Standard Operating Procedure
9. ANNEXES
9.1 Annex-1: Log Book for Operation of FTIR Spectrometer
10. REVISION HISTORY
| Version | Date | Description of Change | Reviewed By | Approved By |
| 1.0 |
Abdus Sobhan Salim is professional experienced pharmacist in pharmaceuticals, author and founder of pharmabossbd.com, the first Bangladeshi pharmaceutical blogger since 2019.
